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dc.contributor.authorSantiago Ortiz, Maricelly
dc.contributor.authorMuñoz Forti, Kevin
dc.contributor.authorSuárez- Martínez, Edu B.
dc.contributor.authorGodoy Muñoz, Lenin
dc.contributor.authorHusain, Kazim
dc.contributor.authorHernández Muñiz, Wilfredo
dc.date.accessioned2017-04-25T19:09:58Z
dc.date.available2017-04-25T19:09:58Z
dc.date.issued2016
dc.identifier.citationInt J Sci: Basic and Appl Res. (2016) 26(1): 26-46. PMID: 27398384.en_US
dc.identifier.issn2307-4531
dc.identifier.urihttp://hdl.handle.net/11721/1588
dc.description.abstractParaquat (PQ) is a commonly used herbicide that induces oxidative stress via reactive oxygen species (ROS) generation. This study aimed to investigate the effects of the antioxidant N-acetylcysteine (NAC) against PQ-induced oxidative stress in mice. Male Balb/C mice (24) were randomly divided into 4 groups and treated for 3 weeks: 1) control (saline), 2) NAC (0.5% in diet), 3) PQ (20 mg/kg, IP) and 4) combination (PQ + NAC). Afterwards mice were sacrificed and oxidative stress markers were analyzed. Our data showed no significant change in serum antioxidant capacity. PQ enhanced lipid peroxidation (MDA) levels in liver tissue compared to control whereas NAC decreased MDA levels (p<0.05). NAC significantly increased MDA in brain tissue (p<0.05). PQ significantly depleted glutathione (GSH) levels in liver (p=0.001) and brain tissue (p<0.05) but non-significant GSH depletion in lung tissue. NAC counteracted PQ, showing a moderate increase GSH levels in liver and brain tissues. PQ significantly increased 8-oxodeoxyguanosine (8-OH-dG) levels (p<0.05) in liver tissue compared to control without a significant change in brain tissue. NAC treatment ameliorated PQ-induced oxidative DNA damage in the liver tissue. PQ significantly decreased the relative mtDNA amplification and increased the frequency of lesions in liver and brain tissue (p<0.0001), while NAC restored the DNA polymerase activity in liver tissue but not in brain tissue. In conclusion, PQ induced lipid peroxidation, oxidative nuclear DNA and mtDNA damage in liver tissues and depleted liver and brain GSH levels. NAC supplementation ameliorated the PQ-induced oxidative stress response in liver tissue of mice.en_US
dc.language.isoenen_US
dc.publisherInternational Journal of Sciences: Basic and Applied Research (IJSBAR)en_US
dc.subjectAntioxidant Capacityen_US
dc.subjectDNA Integrityen_US
dc.subjectGlutathione levelsen_US
dc.subjectMDA levelsen_US
dc.subjectMiceen_US
dc.subjectOxidative Stressen_US
dc.subjectReactive Oxygen Speciesen_US
dc.titleEffects of antioxidant n-acetylcysteine against paraquat 1-induced oxidative stress tissues of mice.en_US
dc.typeArticleen_US
dc.contributor.campusUniversity of Puerto Rico at Ponce


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